期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 106, 期 2, 页码 564-571出版社
WILEY
DOI: 10.1111/j.1365-2672.2008.04026.x
关键词
acetylation; antimicrobial agents; Escherichia coli; fluoroquinolones; quinolone-resistance determining regions; resistance; transformation; wastewater
资金
- Postgraduate Research Fellowship Program
- National Centre for Toxicological Research
- Oak Ridge Institute for Science and Education
- U.S. Department of Energy and the U.S. Food and Drug Administration
To isolate environmental bacteria capable of transforming fluoroquinolones to inactive molecules. Bacteria were isolated from the aerobic liquor of a wastewater treatment plant on a medium containing norfloxacin (100 mg l(-1)). Twenty-two isolates were highly resistant (minimal inhibitory concentration: 6.25-200 mu g ml(-1)) to five fluoroquinolones and six of them were positive by PCR amplification for the aminoglycoside resistance gene aac(6')-Ib. Of these, only Escherichia coli strain LR09 had the ciprofloxacin-acetylating variant gene aac(6')-Ib-cr; HPLC and mass spectrometry showed that this strain transformed both ciprofloxacin and norfloxacin by N-acetylation. This bacterium also had mutations in the quinolone-resistance determining regions of the gyrA and parC genes. An E. coli isolate from wastewater, which possessed at least two distinct fluoroquinolone resistance mechanisms, inactivated ciprofloxacin and norfloxacin by N-acetylation. This is the first report of N-acetylation of fluoroquinolones by an aac(6')-Ib-cr-containing bacterium from an environmental source.
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