期刊
JOURNAL OF APPLIED ICHTHYOLOGY
卷 25, 期 -, 页码 68-74出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1439-0426.2009.01337.x
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资金
- US Army Corporations of Engineers Mississippi Valley Division
P>Thirty-six sturgeon (Scaphirhynchus spp.) were captured from the lower Mississippi River using baited trot-lines, anesthetized using tricaine methanesulfonate, and subjected to endoscopic evaluation of their coelom (coelioscopy) on the river bank. Using a 2.7 mm rod-lens telescope and gravity-fed sterile saline infusion, gender and reproductive stage were determined in the field, and later re-evaluted and confirmed by histology of endoscopic biopsies in the laboratory. Gender and reproductive stage were determined endoscopically in all fish without complication, and proved to correlate 100% with biopsy histology. Modern rod-lens telescopes and sterile saline insufflation can be used under field conditions, provide superior visualization of sturgeon gonads, and offer a high degree of accuracy even with inactive gonads. Rigid endoscopy is recommended as a minimally-invasive means of assessing the reproductive organs of sturgeon and other fish.
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