期刊
JOURNAL OF APPLIED GENETICS
卷 56, 期 1, 页码 15-26出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13353-014-0228-7
关键词
Wheat SOS1 promoter; Na+/H+ antiporter SOS1; Triticum aestivum; Transgenic Arabidopsis; Salt stress; Drought
资金
- Ministry of Higher Education, Scientific Research of Tunisia
- Ministry of Information and Communication Technology of Tunisia
The bread wheat TaSOS1 has been previously shown to be induced by salt stress treatment. To further investigate the regulation of the TaSOS1 gene, the two genomic fragments Pr (SOS1-AB) and Pr (SOS1-D) have been isolated and sequenced. Pr (SOS1-AB) and Pr (SOS1-D) are the promoter regions of SOS1 alleles, which are localised on genomes A and/or B, and on genome D, respectively. Sequence analysis of these two promoters revealed the presence of cis-regulatory elements which could be required for abiotic stress and abscisic acid (ABA) responsiveness. Histochemical assays of stably transformed Arabidopsis T3 plants showed that Pr (SOS1-AB) and Pr (SOS1-D) are active in this heterologous system, and their activities were almost the same at early developmental stages (4-, 8- and 12-day-old transgenic Arabidopsis seedlings). Nevertheless, beta-glucuronidase (GUS) activity was detected only in plants carrying the Pr (SOS1-AB) -gusA construct grown for 20 or 30 days. Furthermore, in these plants, the application of abiotic stress produced an accumulation in gusA transcripts. Taken together, these results show that, in this heterologous dicot system and under normal growth conditions, Pr (SOS1-AB) and Pr (SOS1-D) are age-dependent and organ-specific promoters. However, in the presence of different stress conditions, the activities of these two promoters became different and only Pr (SOS1-AB) is an abiotic stress-inducible promoter at different developmental stages. Thus, Pr (SOS1-AB) can be used for the development of abiotic stress-tolerant transgenic plants.
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