期刊
JOURNAL OF APICULTURAL RESEARCH
卷 52, 期 1, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.3896/IBRA.1.52.1.11
关键词
honey bee; American foulbrood; Paenibacillus larvae; brood; disease; pathogen; technique; BEEBOOK; COLOSS
类别
资金
- Research Foundation of Flanders (FWO-Vlaanderen) [G.0163.11]
- BBSRC [BB/I000801/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I000801/1] Funding Source: researchfish
American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and 'omics and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.
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