期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 68, 期 1, 页码 40-45出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dks365
关键词
MLST; ESBLs; qnr; aac(6)-Ib-cr; topoisomerase IV
资金
- Department of Clinical Microbiology of the Leiden University Medical Center, The Netherlands
To characterize the mechanisms of fluoroquinolone and cephalosporin resistance in Enterobacteriaceae from a Dutch teaching hospital in 2008. We sequenced gyrA, gyrB, parC and parE. The presence of plasmid-encoded genes qnrA, qnrB, qnrS, aac(6)-Ib, qepA, bla(TEM), bla(SHV,) bla(OXA), bla(CTX-M) and bla(AmpC) was studied by PCR. Escherichia coli isolates were further characterized by AFLP and multilocus sequence typing (MLST). In total, 49 E. coli, 16 Klebsiella pneumoniae and 3 Enterobacter cloacae isolates were investigated. Mutations in gyrA were found in all E. coli isolates. Forty-five (92) E. coli isolates carried at least one point mutation in parC. Most E. coli isolates (59) also carried mutations in parE, of which I529L was the most prevalent. I529L was unequivocally associated with E. coli sequence type (ST) 131. This single-nucleotide polymorphism (SNP) was later also found in eight out of nine ST131 strains from another collection. Twenty-nine E. coli isolates carried extended-spectrum -lactamase (ESBL) genes, predominantly bla(CTX-M-15). In E. coli, aac(6)-Ib-cr was the predominant plasmid-mediated resistance mechanism, whereas in K. pneumoniae qnr genes were found mostly. In K. pneumoniae isolates, qnr and aac(6)-Ib-cr co-occurred with ESBL genes (n13; bla(CTX-M) and bla(SHV)) and/or bla(AmpC) (n3; bla(DHA-1)). E. coli ST131 was the predominant clone, which accumulated a high number of chromosomal mutations. The I529L SNP in parE was a signature of most, but not all, ST131 strains. In contrast to E. coli, fluoroquinolone resistance mechanisms were predominantly plasmid-encoded in K. pneumoniae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据