期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 68, 期 4, 页码 766-770出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dks465
关键词
CTX-M; plasmids; animal reservoirs; resistance genes
资金
- National Natural Science Foundation of China [U1031004, 30972218]
Objectives: To investigate the occurrence of plasmid-mediated fosfomycin resistance genes among Escherichia coli from food animals in China. Methods: A total of 892 E. coli isolates collected from individual pigs (n=368), chickens (n=196), ducks (n=261), geese (n=35), pigeons (n=20) and partridges (n=12) in Guangdong Province during 2002-08 were screened for the presence of fosA3, fosA and fosC2 by PCR amplification and sequencing. The clonal relationship of fosA3-positive isolates, plasmid content and other associated resistance genes were also characterized. Results: Twelve (1.3%) E. coli isolates showed resistance to fosfomycin and 10 (1.1%) isolates (4 from pigs, 2 from chickens, 2 from ducks, 1 from a goose and 1 from a pigeon) were positive for fosA3. None of the E. coli isolates was positive for fosA or fosC2. All of the isolates carrying fosA3 were CTX-M producers, and three of them carried rmtB. Most of the fosA3-harbouring isolates were found to be clonally unrelated. The fosA3 genes were flanked by IS26. Two fosA3 genes co-localized with rmtB and bla(CTX-M-65) on indistinguishable F33:A-:B- plasmids that carried three addiction systems (pemI/pemK, hok/mok/sok and srnB). Four, one and one fosA3 genes were found to be associated with IncN (ST8 type), Inch 1 and F2:A-:B- plasmids, respectively. Conclusions: We discovered that fosA3 is always associated with blo(CTX-M), which facilitates its quick dispersal. The emergence of fosA3 in food animals could impact on human medicine by the potential transfer of resistance through the food chain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据