期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 67, 期 8, 页码 1895-1904出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dks132
关键词
antimalarial; immunomodulation; ROS; PPAR; Nrf2
资金
- EU [IP-018834]
- AntiMal International PhD programme, an EMBL collaborative training programme
Curcumin is a natural plant product with antimalarial activity and immunomodulatory properties. In this study we aimed to investigate its effects on CD36 expression and CD36-mediated Plasmodium falciparum phagocytosis as well as the role played by reactive oxygen species (ROS) and the peroxisome proliferator-activated receptor retinoid X receptor (PPAR-RXR) in these processes. In vitro antimalarial activity was evaluated by the [H-3]hypoxanthine assay. ROS production and surface CD36 in human monocyte/macrophages were measured by flow cytometry. PPAR and CD36 mRNA expression was determined by the QuantiGene Plex assay and RT-qPCR. Nuclear PPAR activation was analysed by a DNA-binding ELISA while nuclear erythroid-related factor 2 (Nrf2) expression was analysed by western blotting. P. falciparum phagocytosis was assessed by light microscopy. Curcumins antimalarial activity was confirmed and did not differ between drug-susceptible and -resistant P. falciparum strains. Curcumin increased monocyte ROS production and expression of PPAR and CD36 at the mRNA and protein levels. Although PPAR activation was blocked by the PPAR antagonist GW9662, CD36 expression and CD36-mediated P. falciparum phagocytosis were only inhibited by N-acetylcysteine (NAC), suggesting a PPAR-independent CD36 expression pathway. We then identified seven putative Nrf2 antioxidant response elements on the CD36 gene promoter and showed that NAC inhibited curcumin-induced Nrf2 protein expression. CD36 expression and CD36-mediated P. falciparum phagocytosis by curcumin are dependent on ROS production and probably involve the Nrf2 pathway. The dual immunomodulatory and antimalarial mechanisms of curcumin action may mean that curcumin has potential as an adjuvant treatment limiting the risk of recrudescence following standard antimalarial therapy.
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