期刊
JOURNAL OF ANTIBIOTICS
卷 66, 期 1, 页码 37-41出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ja.2012.83
关键词
cis-prenyltransferase; methicillin-resistant Staphylococcus aureus; spirohexaline; undecaprenyl pyrophosphate
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [18390008]
- Uehara Memorial Foundation
An enzyme assay for bacterial undecaprenyl pyrophosphate (UPP) synthase was performed to screen microbial culture broths for inhibitors of UPP synthase. During the course of this screening program, an EtOH extract of a rice culture of Penicillium brasilianum FKI-3368 was found to inhibit UPP synthase activity. From activity-guided purification, a new compound-designated spirohexaline was isolated together with the structurally related and known viridicatumtoxin by ethyl acetate extraction silica gel and octadecylsilane column chromatographies and high-performance liquid chromatography. The structure of spirohexaline was elucidated by spectroscopic analysis, including NMR. Spirohexaline and viridicatumtoxin have a common hexacycline structure produced by fusion of a tetracycline-type ring with a spiro-type ring. They inhibited UPP synthase activity with IC50 values of 9.0 and 4.0 mu M, respectively. The Journal of Antibiotics (2013) 66, 37-41; doi: 10.1038/ja.2012.83; published online 21 November 2012
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