The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae

Two distinct metabolic pathways have been elucidated for the formation of isopentenyl diphosphate and dimethylallyl diphosphate, essential metabolic precursors for isoprenoid biosynthesis the mevalonate pathway, found ubiquitously in mammals, and the 2 C-methyl D erythritol 4 phosphate (MEP) pathway, found in most bacteria As the MEP pathway is absent from mammals, all MEP pathway enzymes represent effective targets for the development of antibacterial drugs In this study, we found that a herbicide, ketoclomazone, exhibited antibacterial activity against a pathogenic bacterium, Haemophilus influenzae, with an MIC value of 12 5 mu g ml(-1) and that antibacterial activity was suppressed by adding 1 deoxyxylulose, a free alcohol of 1 deoxy D-xylulose 5 phosphate (DXP) DXP is an MEP pathway intermediate synthesized from pyruvate and D glyceraldehyde 3 phosphate (D-GAP) by the action of DXP synthase Thus, we investigated the enzyme kinetics of DXP synthase of H influenzae (HIDXS) to elucidate an inhibitory mechanism of ketoclomazone on HIDXS The dxs gene was cloned from H influenzae and overexpressed in Escherichia coli, and the enzyme was purified to homogeneity The purified HIDXS was a soluble dimeric 70-kDa protein Steady-state kinetic constants for HIDXS were calculated, and Lineweaver-Burk plots were consistent with a ping pong bi bi mechanism The kinetics of inhibition by ketoclomazone suggested that ketoclomazone binds to an unidentified inhibitor binding site that differs from both the pyruvate binding site and the D-GAP binding site on DXP synthase These data reveal the inhibitory mechanism of ketoclomazone on DXP synthase The Journal of Antibiotics (2010) 63, 583-588, doi 10 1038/ja 2010 100 published online 1 September 2010