期刊
JOURNAL OF ANIMAL SCIENCE
卷 92, 期 3, 页码 1068-1073出版社
AMER SOC ANIMAL SCIENCE
DOI: 10.2527/jas.2013-6485
关键词
breast muscle; broiler; methionine; methylation; myogenic gene expression
To investigate the mechanism underlying the regulatory effect of Met on broiler growth, the growth performance, organ development, serum profile, myogenic gene expression, and methylation of myostatin gene exon 1 region in response to dietary Met status were evaluated. A total of 192 one-day-old Arbor Acres broiler chicks were housed in 3-layer cages in a temperature-controlled room with continuous lighting. The temperature of the room was maintained at 32 to 34 degrees C for the first 3 d and then reduced by 2 to 3 degrees C per week to a final temperature of 20 degrees C. Cages were randomly allocated to 2 dietary treatments with 6 replicate cages (8 males and 8 females/ cage) per treatment. Control starter and finisher diets contained 0.50 and 0.43% Met, respectively. Corresponding values for a + Met treatment were 0.60 and 0.53% Met, respectively. The birds receiving the +Met diets had a greater (P < 0.05) G: F throughout the experiment. The +Met diets increased (P < 0.05) the relative weight of breast muscle and the concentrations of uric acid and triglyceride in serum at 42 d of age, whereas other serum measurements were not affected by treatments. Increased myogenic factor 5 (Myf5) and myocyte enhancer factor 2B (MEF2B) and decreased myostatin mRNA expression were observed in broilers fed the +Met diets (P < 0.05). However, methylation of myostatin gene exon 1 region was not different between groups. In conclusion, broilers fed the +Met diets increased breast muscle growth that was reflected in the expected expression of myostatin, Myf5, and MEF2B genes.
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