期刊
ACTA BIOMATERIALIA
卷 22, 期 -, 页码 39-49出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.04.036
关键词
Skin; Wound healing; Adipose-derived stem cells; Adipocytes; Tissue engineering
资金
- Fondation du CHU de Quebec
- Canadian Institutes of Health Research (CIHR) [111233]
- Centre de recherche du CHU de Quebec du Fonds de la recherche du Quebec - Sante (FRQ-S)
- Reseau Thecell du FRQ-S
- CIHR
- FRQ-S
Promotion of skin repair for acute or chronic wounds through the use of tissue-engineered products is an active field of research. This study evaluates the effects mediated by tissue-engineered biological dressings containing human in vitro-differentiated adipocytes and adipose-derived stromal cells (ASCs). Re-epithelialization, granulation tissue formation and neovascularization of full-thickness cutaneous wounds were specifically assessed using a murine model featuring a fluorescent epidermis. In comparison with wounds that did not receive an adipocyte-containing biological dressing, treated wounds displayed a slight but significantly faster wound closure based on macroscopic observations over 18 days. Non-invasive imaging of GFP-expressing keratinocytes determined that the kinetics of re-epithelialization were similar for both groups. Treated wounds featured thicker granulation tissues (1.7-fold, P < 0.0001) enriched in collagens (1.3-fold, P < 0.0104). In addition, wound cryosections labeled for detection of CD31-expressing cells indicated a 2.2-fold (P < 0.0002) increased neovascularization for the treated wounds at the time of terminal biopsy. This is in accordance with the secretion of pro-angiogenic factors detected in media conditioned by the dressings. Taken together, these results establish that a new type of engineered substitutes featuring a mixture of adipocytes and ASCs can promote cutaneous healing when applied as temporary dressings, suggesting their potential relevance for chronic wound management studies. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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