期刊
JOURNAL OF ANIMAL SCIENCE
卷 88, 期 2, 页码 421-427出版社
AMER SOC ANIMAL SCIENCE
DOI: 10.2527/jas.2009-1955
关键词
oviduct; PGR genotype; progesterone receptor expression; rabbit; uterus
资金
- MCYT [AGL2001-3068-C03, AGL2005-07624-C03]
The association of the 2464G > A SNP found in the promoter region of the rabbit progesterone receptor gene with progesterone receptor ( PR) expression was evaluated by Western blot analysis. This SNP was associated with 2 lines divergently selected for uterine capacity, the high line selected to increase uterine capacity and the low line selected to decrease uterine capacity. Two progesterone isoforms were obtained using a commercial monoclonal antibody: the PR-B isoform described previously in rabbits, and the PR-A isoform, not described previously in rabbits. The GG genotype, the genotype more frequent in the high line, showed less PR-B and PR-A expression than the AA genotype in the oviduct (GG/AA(PR-B) = 0.81 and GG/AA(PR-A) = 0.73) and uterus ( around 0.70 in both isoforms). The GA genotype showed similar PR-A expression in both tissues and also similar PR-B expression in the oviduct to the GG genotype. Conversely, the GG genotype showed less PR-B expression than the GA genotype in the uterus (GG/GA(PR-B) = 0.79). Similar expression of both PR isoforms was found in the uterus at d 2 and 3 of gestation; meanwhile, an increase of both isoforms was observed in the oviduct. Similar PR-A expression was observed in the ampulla and isthmus; meanwhile, the PR-B expression in the isthmus was double that in the ampulla.
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