期刊
NANOTECHNOLOGY
卷 26, 期 35, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/26/35/355501
关键词
single-cell temperature measurement; temperature measurement probe; Seebeck coefficient; precision; marine lung epithelia (MLE)-12 cells
资金
- National Key Basic Research Program of China [2011CB933503]
- International Cooperation and Communication Program of China [61420106012]
In this work, a nanoprobe that is highly thermo-sensitive to tiny temperature changes was prepared based on a thermocouple metal junction. A series of electro-element apparatuses were integrated to accomplish single-cell temperature measurement. The temperature measurement probe (TMP) was constructed by tungsten (W), polyurethane (PU), and platinum (Pt). The tip size of TMP was characterized at less than 500 nm, and the tip angle was between 10 and 20 degrees with the resistance in the range of 500 to 1500 Omega. The single-cell temperature measurement probes were calibrated and calculated with a Seebeck coefficient ranging from 6 to 8 mu V degrees C-1 at a precision of 0.1 degrees C. Monitoring the temperature at a single-cell level by inserting the TMP in marine lung epithelia (MLE)-12 cells displayed that the stimulation of lipopolysaccharide (LPS) and cobalt chloride induced different single-cell temperature fluctuation. This investigation could help reveal complex cellular functions and develop novel diagnoses.
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