期刊
JOURNAL OF ANALYTICAL CHEMISTRY
卷 68, 期 11, 页码 1007-1013出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1061934813110087
关键词
second-derivative spectrophotometry; vitamin B-3; cosmetic preparations; TLC; HPLC
Differential spectrophotometry and liquid chromatography (TLC, HPLC) were used to qualitative and quantitative determination of nicotinamide (vitamin B-3) in commercial cosmetics creams. The liquid-liquid extraction has been used as pretreatment processes for cosmetics samples prior to their analyses. Differential spectra were collected using a UV-Vis spectrophotometer with the range of 220-350 nm, in a 1-cm layer, with the speed of spectrum registering at 240 nm/min, the spectra width of the radiation leaving the monochromator of 1.00 nm and the increase in wavelength of 1 nm. The measuring methods of peak-zero and peak-peak were used. The reversed-phase determination of vitamin B-3 was conducted on a LiChrospher 100 RP-18 column (250 x 4 mm, 10 mu m) at 260 nm, using a mixture of KH2PO4 (0.02 M)-methanol (9: 1) as a mobile phase (the flow rate - 1.5 mL/min). The UV method (D-2) for the determination of vitamin B-3 in cosmetic products is accurate with the comparison of reference method of HPLC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据