期刊
JOURNAL OF ALZHEIMERS DISEASE
卷 29, 期 4, 页码 921-930出版社
IOS PRESS
DOI: 10.3233/JAD-2012-112057
关键词
Adult neurogenesis; dentate gyrus; subgranular zone; tau protein
资金
- JAEDoc Postdoctoral program
- Spanish Ministry of Health [SAF 2006-02424, SAF2010-15525]
- Comunidad de Madrid [SAL/0202/2006]
- Fundacion CIEN [PI 008-09]
- Fundacion M. Botin
- Fundacion R. Areces
In the adult hippocampal dentate gyrus, newborn granule cells grow dendrites into the molecular layer and send axons into the CA3 region. Several molecular markers have been used to analyze production of these new neurons; however, no good markers for new axons have been described. Here we demonstrate that tau protein isoform with three microtubule binding domains (3R-Tau) is a marker of those axons following an antigen retrieval protocol. By using retrovirus-mediated GFP transduction, GFP can be detected in a period of 7-14 days after viral infection. We also provide a proof of principle demonstration of the power of that labeling showing modulation of 3R-Tau positive axons under physiological conditions (exercise and aging) as well as in a FTDP-17 neurodegenerative model and Alzheimer's disease models (mice overexpressing A beta PPsw, ind or GSK3 beta). We conclude that 3R-Tau would be an efficient marker and a valuable tool to study new axons in adult neurogenesis as well as in neurodegenerative processes.
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