期刊
NANOSCALE
卷 7, 期 9, 页码 3867-3875出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4nr04759a
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- Association Francaise contre les Myopathies
- Ministry of Science and Technology of China [2012AA022501]
- Agence National de la Recherche
- Canceropole PACA
- INCa
- Wuhan University
- Aix-Marseille University
- CNRS
- INSERM
- China Scholarship Council
- international ERA-Net EURONANOMED European Research project DENANORNA
- European COST Action [TD0802]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL074704] Funding Source: NIH RePORTER
RNA interference (RNAi) with small interfering RNA (siRNA) is expected to offer an attractive means to specifically and efficiently silence disease-associated genes for treating various diseases provided that safe and efficient delivery systems are available. In this study, we have established an arginine-decorated amphiphilic dendrimer composed of a hydrophobic alkyl chain and a hydrophilic PAMAM dendron bearing arginine terminals as nonviral vector for siRNA delivery. Indeed, this dendrimer proved to be very effective at delivering siRNAs in human prostate cancer PC-3 cells and in human hematopoietic CD34+ stem cells, leading to improved gene silencing compared to the corresponding nonarginine decorated dendrimer. Further investigation confirmed that this dendrimer was granted with the capacity to form stable nanoparticles with siRNA and significantly enhance cellular uptake of siRNA. In addition, this dendrimer revealed no discernible cytotoxicity. All these findings demonstrate that decoration of the dendrimer surface with arginine residues is indeed a useful strategy to improve the delivery ability of dendrimers.
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