期刊
NANOSCALE
卷 7, 期 7, 页码 3300-3308出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4nr06664b
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资金
- National Natural Science Foundation of China [21371007]
- Anhui Provincial Natural Science Foundation [1208085QB28]
- Anhui Provincial Natural Science Foundation for Distinguished Youth [1408085J03]
- Natural Science Foundation of Anhui [KJ2012A139]
- Program for Innovative Research Team at Anhui Normal University
In the present study, based on the mimic oxidase catalytic character of nucleic-acid-stabilized silver nanoclusters (DNA/AgNCs) and hybridization chain reactions for signal amplification, the fabrication of a label-free sensitive turn-on electrochemical aptasensor for the amplified determination of lysozyme was demonstrated. First, the designed DNA duplex was modified on the electrode. With the specific binding of the target, lysozyme and its aptamer, the lysozyme-binding DNA sequence was liberated, exposing the induced DNA sequence, which in turn triggered the formation of the supersandwich DNA structure. Because the cytosine-rich sequence was designed ingeniously on the DNA sequence, DNA/AgNCs were formed on the supersandwich DNA structure. The peroxidase-like character of DNA/AgNCs produced detectable electrochemical signals for the lysozyme aptasensor, which showed a satisfying sensitive detection of lysozyme with a low detection limit of 42 pM and a wide linear range of 10(-10) M to 10(-5) M.
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