期刊
NANOSCALE
卷 7, 期 40, 页码 16578-16582出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr05716g
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资金
- National Natural Science Foundation of China [21274111, 51473123, 51402215]
- Recruitment Program of Global Experts
- China Postdoctoral Science Foundation [2014M550245]
- Shanghai Postdoctoral Science Foundation [14R21411200]
This study describes a new strategy for the fabrication of magnetic core-shell microgels by free-radical polymerization triggered by the cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP). The mild polymerization around the interface of the magnetic nanoparticles permits the mild coating of the microgel layer with excellent characteristics for various applications in biocatalysis and medical diagnostics, as well as in clinical fields. The immobilized bienzyme within the microgel has a largely retained activity relative to the non-immobilized one. The confining effect of the microgel and the well designed distance between the two enzymes can benefit the diffusion of intermediates to the HRP active site. The final microgels can be incontestably employed as sensitive biosensors for colorimetric glucose detection.
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