期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 11, 期 6, 页码 1521-1530出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2015.03.013
关键词
Resveratrol; Quercetin; Epicatechin; Cortical actin; Atomic force microscopy; Cell cortex
资金
- Deutsche Forschungsgemeinschaft [OB 68/1, KU 1496/7-1]
- Else-Kroner-Fresenius Stiftung [2010_A116]
- Innovative Medical Research (IMF) of the University of Munster [KU 120808]
- Centre of Excellence (Cells in Motion
- CIM), University of Munster
Previous studies show that polyphenol-rich compounds can induce a swelling of the endothelial glycocalyx (eGC). Our goal was to reveal the mechanism behind the eGC-swelling. As polyphenols are potent modulators of fibrosis transmembrane conductance regulator (CFTR) Cl-channel, the hypothesis was tested whether polyphenol-induced increase in CFTR activity is responsible for the eGC-swelling. The impact of the polyphenols resveratrol, (-)-epicatechin, and quercetin on nanomechanics of living endothelial GM7373 cells was monitored by AFM-nanoindentation. The tested polyphenols lead to eGC-swelling with a simultaneous decrease in cortical stiffness. EGC-swelling, but not the change in cortical stiffness, was prevented by the inhibition of CFTR. Polyphenol-induced eGC-swelling could be mimicked by cytochalasin D, an actin-depolymerizing agent. Thus, in the vascular endothelium, polyphenols induce eGC-swelling by softening cortical actin and activating CFTR. Our findings imply that CFTR plays an important role in the maintenance of vascular homeostasis and may explain the vasoprotective properties of polyphenols. (C) 2015 The Authors. Published by Elsevier Inc.
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