期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 124, 期 5, 页码 990-996出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2009.08.039
关键词
Asthma; children; nitric oxide; nitrogen oxides; nitrosation; nitrosative stress; reactive nitrogen species
资金
- National Institutes of Health/National Institute of Nursing Research [KO1 NR010548]
- National Institutes of Health/National Center for Research Resources [K 12 RR017643]
- National Institutes of Health/National Heart, Lung, and Blood Institute Severe Asthma Research [RO1 HL69170]
Background: Children with severe allergic asthma have persistent airway inflammation and oxidant stress. Objectives: We hypothesized that children with severe allergic asthma would have increased concentrations of the nitric oxide (NO) oxidation products nitrite, nitrate, and nitrotyrosine in the proximal and distal airway epithelial lining fluid (ELF). We further hypothesized that NO oxidation products would be associated with higher exhaled NO values (fraction of exhaled nitric oxide [F-ENO]), greater allergic sensitization, and lower pulmonary function. Methods: Bronchoalveolar lavage fluid was obtained from 15 children with mild-to-moderate asthma, 30 children with severe allergic asthma, 5 nonasthmatic children, and 20 nonsmoking adults. The bronchoalveolar lavage fluid was divided into proximal and distal portions and nitrite, nitrate, and nitrotyrosine values were quantified. Results: Children with mild-to-moderate and severe allergic asthma had increased concentrations of nitrite (adult control subjects, 15 +/- 3 mu mol/L; pediatric control subjects, 23 +/- 4 mu mol/L; subjects with mild-to-moderate asthma, 56 +/- 26 mu mol/L; subjects with severe asthma, 74 +/- 18 mu mol/L), nitrate (37 +/- 13 vs 145 +/- 38 vs 711 +/- 155 vs 870 +/- 168 mu mol/L, respectively) and nitrotyrosine (2 +/- 1 vs 3 +/- 1 vs 9 +/- 3 vs 10 +/- 4 mu mol/L, respectively) in the proximal ELF. Similar results were seen in the distal ELF, although the concentrations were significantly lower (P < .05 for each). Although univariate analyses revealed no associations between NO oxidation products and clinical features, multivariate analyses revealed F-ENO values to be a significant predictor of NO oxidation in asthmatic children. Conclusions: NO oxidation products are increased in the ELF of asthmatic children. The relationship between F-ENO values and airway nitrosative stress is complicated and requires further study. (J Allergy Clin Immunol 2009;124:990-6.)
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