期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 123, 期 1, 页码 67-73出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2008.09.049
关键词
CCR4; CCR8; CXCR3; asthma; chemokine; T-cell trafficking
资金
- National Institutes of Health [R37-AI40618, K08AI67519]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R37AI040618, K08AI067519, R01AI040618] Funding Source: NIH RePORTER
Background: Recruitment of antigen-specific T(H)2 cells into the lung is critical for the development of allergic airway inflammation. Although CCR4 and CCR8 are preferentially expressed on T(H)2 cells and CCR4, CCR8, and CXCR3 ligands are increased in asthma, the specific relative contribution of these receptors to antigen-specific T(H)2 cell trafficking into the allergic lung is not known. Objective: To determine the relative contribution of the chemokine receptors CCR4, CCR8, and CXCR3 to antigen-specific T(H)2 cell trafficking in a murine model of allergic pulmonary inflammation. Methods: We used adoptive transfer experiments to compare the trafficking of wild-type antigen-specific T(H)2 cells with antigen-specific T(H)2 cells deficient in CCR4, CCR8, or CXCR3. Results: CCR4-deficient antigen-specific T(H)2 cells failed to traffic efficiently into the lung and the airways. In contrast, CCR8-deficient antigen-specific T(H)2 cells accumulated in these sites. Trafficking of CXCR3-deficient antigen-specific T(H)2 cells and CCR4-deficient and CCR8-deficient antigen-specific Till cells were comparable to their wild-type counterparts. Approximately 60% of IL-4-producing antigen-specific T cells expressed CCR4. Disruption of CCR4-mediated antigen-specific T(H)2 cell trafficking decreased the levels of T(H)2-type cytokines in the airways and reduced airway eosinophilia and mucus production. Conclusions: Our study demonstrates that CCR4 is required for file efficient entry of antigen-specific T(H)2 cells into the lung and the airways in a murine model of allergic pulmonary inflammation. (J Allergy Clin Immunol 2009;123:67-73.)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据