期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 121, 期 4, 页码 1020-1026出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2007.11.031
关键词
mast cells; proteoglycans; serglycin; histamine; serotonin; histidine decarboxylase; tryptophan hydroxylase
Background: Serotonin and histamine are components of human and rodent mast cell secretory granules. Objective: Serotonin and histamine are stored in the same compartment as serglycin proteoglycan. Here we addressed the possibility that serglycin may be involved in their storage and/or release. Methods: The storage and release of histamine and serotonin was studied in bone marrow-derived mast cells (BMMCs) and in peritoneal mast cells from wild-type or serglycin(-/-) mice. Results: Both serotonin and histamine storage in BMMCs was positively correlated with the degree of mast cell differentiation, and the amount of stored amine was reduced in serglycin(-/-) BMMCs compared with wild-type controls. The amounts of histamine/serotonin stored were reflected by the expression levels of histidine decarboxylase and tryptophan hydroxylase 1, respectively. Calcium ionophore activation resulted in serotonin/histamine release both from wild-type and serglycin(-/-) BMMCs. Interestingly, serotonin release was induced in cells lacking intracellular stores of serotonin, suggesting de novo synthesis. The knockout of serglycin affected the levels of stored and released mast cell serotonin and histamine to an even larger extent in in vivo-derived mast cells than in BMMCs. Conclusion: These results establish a previously assumed, but not proven, role of serglycin in storage of histamine and, further, establish for the first time that serotonin storage in mast cells is dependent on serglycin proteoglycan.
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