期刊
NANO LETTERS
卷 15, 期 5, 页码 2765-2772出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl502227a
关键词
silicon nanowires; cardiac spheroids; cardiomyocytes; human induced pluripotent stem cells; maturation
类别
资金
- National Institutes of Health [8P20 GM103444, U54 GM104941, HL 085847, R01HL095696, R01HL094545]
- Clemson University
- National Science Foundation [NSF - EPS-0903795]
- NIH [T32 HL007260]
- Veterans' Affairs Health Administration [BX002327]
- NIH-NIGMS [P30 GM103342]
- Office Of The Director
- Office of Integrative Activities [1317771] Funding Source: National Science Foundation
The current inability to derive mature cardiomyocytes from human pluripotent stem cells has been the limiting step for transitioning this powerful technology into Clinical therapies. To address this, scaffold-based tissue engineering approaches have been utilized to mimic heart development in vitro and promote maturation of cardiomyocytes derived from human pluripotent stem cells. While scaffolds can provide 3D microenvironments, current scaffolds lack the matched physical/chemical/biological properties of native extracellular environments. On the other hand, scaffold-free, 3D cardiac spheroids (i.e., spherical-shaped microtissues) prepared by seeding cardiomyocytes into agarose microwells were shown to improve cardiac functions. However, cardiomyocytes within the spheroids could not assemble in a controlled manner and led to compromised, unsynchronized contractions. Here, we show, for the first time, that incorporation of a trace amount (i.e. similar to 0.004% w/v) of electrically conductive silicon nanowires (e-SiNWs) in otherwise scaffold-free cardiac spheroids can form an electrically conductive network, leading to synchronized and significantly enhanced contraction (i.e., >55% increase in average contraction amplitude), resulting in significantly more advanced cellular structural and contractile maturation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据