期刊
NANO LETTERS
卷 16, 期 1, 页码 381-386出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b03956
关键词
Magnetic tweezers; FRET; single-molecule; correlated measurements; force-fluorescence microscopy
类别
资金
- ERC [261224]
- German Ministry for Science and Education [BMBF 03Z2EN11]
- European Research Council (ERC) [261224] Funding Source: European Research Council (ERC)
We present a hybrid single-molecule technique combining magnetic tweezers and Forster resonance energy transfer (FRET) measurements. Through applying external forces to a paramagnetic sphere, we induce conformational changes in DNA nanostructures, which are detected in two output channels simultaneously. First, by tracking a magnetic bead with high spatial and temporal resolution, we observe overall DNA length changes along the force axis. Second, the measured FRET efficiency between two fluorescent probes monitors local conformational changes. The synchronized orthogonal readout in different observation channels will facilitate deciphering the complex mechanisms of biomolecular machines.
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