期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 42, 页码 11043-11054出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b04001
关键词
activated sludge; polyhydroxyalkanoates; substrate; acclimation time; microbial community
资金
- National Natural Science Foundation of China [41571449, 41807035]
- Program for New Century Excellent Talents in University [NCET-12-0326]
- Natural Science Foundation of Fujian Provincial University Youth Key Program of China [JZ160401]
- Natural Science Foundation of Fujian Province of China [2018J05073, 2018Y0074]
The effects of octanoic acid/nonanoic acid and acclimation time on the synthesis of short-chain-length and medium-chain-length PHA blends from activated sludge were investigated. An increased concentration (847-1366 mg/L) of PHAs resulted from 4-month acclimation compared with the concentration derived from 2-month acclimation (450-1126 mg/L). The content of octanoic acid had a positive linear relationship with the content of even-numbered carbon monomers among the PHAs. The blending products were identified mainly with scl-PHAs during the 2-month acclimation period and were thereafter dominated by mcl-PHAs until 4 months of acclimation. Thermal properties analysis demonstrated that the products derived from 4-month acclimation were a mixture of scl-PHAs and mcl-PHAs rather than a copolymer of scl-PHAs and mcl-PHAs. High-throughput sequencing results indicated that Pseudofulvimonas, Paracoccus, and Blastocatella were the dominant genera that might be responsible for scl-PHAs production during the 2-month acclimation period, whereas Comamonas and Pseudomonas that were responsible for mcl-PHAs production then became the dominant genera after 4-months acclimation.
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