4.7 Article

Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 37, 页码 9624-9634

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b03054

关键词

Alopecurus aequalis Sobol.; acetyl-CoA carboxylase; acetolactate synthase; quantitative real-time PCR; endogenous reference gene; gene amplification

资金

  1. National Natural Science Foundation of China [31772181, 31601653]
  2. National Key R&D Program of China [2016YFD0300709, 2016YFD0300701]
  3. Funds of Shandong Double Tops Program [SYL2017XTTD11]
  4. Special Fund for Agro-Scientific Research in the Public Interest [201303031]

向作者/读者索取更多资源

Severe infestations of shortawn foxtail (Alopecurus aequalis Sobol.), a noxious weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop-P-ethyl and mesosulfuron-methyl. Analysis of gene expression in weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

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