期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 20, 页码 4661-4667出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf500189a
关键词
corn cob; deacetylation; PFI refining; enzymatic hydrolysis; pretreatment; xylo-oligosaccharides (XOS)
资金
- Natural Science Foundation of China [21206184, 31370582, 21306216]
- Shandong Provincial Natural Science Foundation for Distinguished Young Scholar (China) [JQ201305]
- National High Technology Research and Development Program (863 Program) of China [2012AA022301]
A combined deacetylation and PFI refining pretreatment was applied to corn cob for the improvement of a two-stage enzymatic hydrolysis. In stage 1, the pretreated corn cob was first hydrolyzed by xylanase to produce xylo-oligosaccharides (XOS). In stage 2, the solid residue isolated from stage 1 was further hydrolyzed by cellulase and beta-glucosidase. NaOH, Na2CO3, and Ca(OH)(2) were tested to remove acetyl groups in the process of deacetylation, and it was found that Ca(OH)(2) could be the most suitable alkali for deacetylation in this work. After deacetylation using 0.8 mmol of Ca(OH)(2)/g of substrate and PFI refining, 50.5% xylan in the raw material could be hydrolyzed into XOS. The corresponding xylan yield of stage 1, the glucan yield of stage 2, and the total sugar yield (all sugars released in the hydrolyzate) after the two-stage enzymatic hydrolysis were 0.306, 0.305, and 0.661 g/g of corn cob, respectively.
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