期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 22, 页码 5142-5148出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf500744n
关键词
diglycerides; phytosterol esters; functional oil; enzymatic transesterification
资金
- National Science Foundation of China [NSFC-31371843, 31101353]
- Director Fund of Oil Crops Research Institute [1610172014006]
- Open Foundation of Hubei Key Laboratory of Lipid Chemistry and Nutrition [2012003]
- Foundation for Modem Agro-industry Technology Research System [CARS-13]
Diglycerides and phytosterol esters are two important functional lipids. Phytosterol esters mixed with dietary diglyceride could not only influence body weight but also prevent or reverse insulin resistance and hyperlipidemia. In this study, a kind of novel functional oil rich in both diglycerides and phytosterol esters was prepared with one-pot enzymatic transesterification. First, lipase AYS (Candida rugosa) was immobilized on the porous cross-linked polystyrene resin beads (NKA) via hydrophobic interaction. The resulting immobilized AYS showed much better transesterification activity and thermal stability to freeways. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different triglycerides to produce corresponding functional oils rich in both diglycerides and phytosterol esters was developed. Four functional oils rich in both diglycerides and phytosterol esters with conversions >92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mmol/L phytosterols, 160 mmol/L triglycerides, and 25 mg/mL AYS@NKA at 180 rpm and 50 degrees C for 12 h in hexane. The prepared functional oil possessed low acid value (<= 1.0 mgKOH/g), peroxide value (<= 2.1 mmol/kg), and conjugated diene value (<= 1.96 mmol/kg) and high diglyceride and phytosterol ester contents (>= 10.4 and >= 20.2%, respectively). All of the characteristics favored the wide application of the functional oil in different fields of functional food.
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