期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 21, 页码 4870-4877出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf404792z
关键词
silver(I) ions; detection; fluorescent oligonucleotide probe; hexachloro fluorescein; G-quadruplex
资金
- National Natural Science Foundation of China [21075097]
- Key Program for Science and Technology Innovative Research Team of Shaanxi Province [2013KCT-24]
Due to the high toxicity of silver(I) ions, a method for the rapid, sensitive, and selective detection for silver(1)ions in water, pharmaceutical products, and food is of great importance. Herein, a novel single-labeled fluorescent oligonucleotide (OND) probe based on cytosine-Ag(I) cytosine coordination and the inherent fluorescence quenching ability of the G-quadruplex is designed to detect silver(I) ions. The formation of a hairpin structure in the OND-Ag(I) complex brings the hexachloro fluorescein (HEX) labeled at the 5'-end of the OND probe close to the G-quadruplex located at the 3'-end of the OND probe, leading to a fluorescence quenching due to photoinduced electron transfer between HEX and the G-quadruplex. Through this method, silver(I) ions can be detected quantitatively, the linear response range is from 1 to 100 nmol/L with a detection limit of 50 pmol/L, and no obvious interference occurs with other metal ions with a 10-fold concentration. This assay is simple, sensitive, and selective, and it can be used to detect silver(I) ions in actual water, drug, and food samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据