期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 19, 页码 4466-4474出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf500854u
关键词
aflatoxin; surface-enhanced Raman spectroscopy (SERS); nanostructure; chemometrics; food safety
资金
- Andersons Endowment
- National Corn Growers Association (Aflatoxin Mitigation Center of Excellence Research Program)
Rapid and sensitive surface-enhanced Raman spectroscopy (SEAS) for aflatoxin detection was employed for development of the models to classify and quantify aflatoxin levels in maize at concentrations of 0 to 1,206 mu g/kg. Highly effective SERS substrate (Ag nanosphere) was prepared and mixed with a sample extract for SERS measurement. Strong Raman bands associated with aflatoxins and changes in maize kernels induced by aflatoxin contamination were observed in different SERS spectroscopic regions. The k-nearest neighbors (KNN) classification model yielded high classification accuracy and lower prediction error with no misclassification of contaminated samples as aflatoxin negative. The multiple linear regression (MLR) models showed a higher predictive accuracy with stronger correlation coefficients (r = 0.939-0.967) and a higher sensitivity with lower limits of detection (13-36 mu g/kg) and quantitation (44-121 mu g/kg) over other quantification models. Paired sample t test exhibited no statistically significant difference between the reference values and the predicted values of SEAS in most chemometric models. The proposed SERS method would be a more effective and efficient analytical tool with a higher accuracy and lower constraints for aflatoxin analysis in maize compared to other existing spectroscopic methods and conventional Raman spectroscopy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据