期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 1, 页码 290-296出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf503351W
关键词
Streptomyces; geosmin; antifungal activity; heptaene macrolide antibiotics; Chinese liquor
资金
- National High Technology Research and Development Program of China [2013AA102108, 2012AA021301]
- National Natural Science Foundation of China [31371822, 31271921]
- Program of Introducing Talents of Discipline to Universities (111 Project) [111-2-06]
- Cooperation Project of Jiangsu Province among Industries, Universities, and Institutes [BY2010116]
- Fundamental Research Funds for the Central Universities [JUDCF10016]
Diverse Streptomyces species act as geosmin producers in the Chinese liquor-making process, causing an earthy, off-odor containment. Through microbiological and metabolite analyses, this paper investigates the influence of several geosmin-producing Streptomyces on the microbial community of a brewing system. The antifungal activity against functional liquor-brewing microbes was assayed by an agar diffusion method. Several Streptomyces, most notably Streptomyces sampsonii QC-2, inhibited the growth of the brewing functional yeasts and molds in pure culture. In a simulated coculture, Streptomyces spp. reduced the flavor compounds (alcohols and esters) contributed by yeasts. Nine components in Streptomyces sampsonii QC-2 broth were detected by ultraperformance liquid chromatography coupled with photo diode array (UPLC-PDA), with characteristic ultraviolet absorptions at 360, 380, and 400 nm. The main products of Streptomyces sampsonii QC-2 were identified by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS), and confirmed by standard mass spectrometry. The antifungal active components were revealed as a series of heptaene macrolide antibiotics.
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