期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 24, 页码 5555-5564出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf5006269
关键词
lactic acid bacteria (LAB); Enterococcus faecalis; gelatinase (GelE); serine protease (SprE); ACE-inhibitory peptides; antihypertensive peptides
资金
- Ministerio de Economia y Competitividad (MINECO) [AGL2012-34829]
- Ministerio de Ciencia e Innovacion (MICINN) [AGL2009-08348, AGL2011-24643]
- CENIT project from the MITC-CDTI
- BSCH-UCM [GR35-10A]
- Comunidad de Madrid (CAM) [S2009/AGR-1489, P2013/ABI-2747]
- Ministerio de Educacion y Ciencia (MEC)
- CAM
- MICINN, Spain
In this work, genes encoding gelatinase (gelE) and serine proteinase (sprE), two extracellular proteases produced by Enterococcus faecalis DBH18, were cloned in the protein expression vector pMG36c, containing the constitutive P-32 promoter, generating the recombinant plasmids pCG, pCSP, and pCGSP encoding gelE, sprE, and gelE sprE, respectively. Transformation of noncaseinolytic E. faecalis P36, E. faecalis JH2-2, E. faecium AR24, and E. hirae AR14 strains with these plasmids permitted detection of caseinolytic activity only in the strains transformed with pCG or pCGSP. Complementation of a deletion (knockout) mutant of E. faecalis V583 for production of gelatinase (GelE) with pCG unequivocally supported that gelE is responsible for the caseinolytic activity of the transformed strain grown in bovine skim milk (BSM). RP-HPLC-MS/MS analysis of hydrolysates of transformed Enterococcus spp. strains grown in BSM permitted the identification of 38 major peptide fragments including peptides with previously reported angiotensin converting enzyme-inhibitory activity (ACE-IA), antihypertensive activity, and antioxidant activity.
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