期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 26, 页码 6138-6146出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf5001138
关键词
Pseudomonas monteilii; 2, 4-di-tert-butylphenol; Fusarium oxysporum; molecular modeling; docking; beta-tubulin
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
A novel strain of Pseudomonas monteilii, PsF84, was isolated from tannery waste soil from Jajmau, Kanpur, India. 16S rRNA gene sequence phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as P. monteilii. An antifungal volatile organic compound (VOC) active against hyphal growth of Fusarium oxysporum (CIMAP-IMI-357464) in vitro was isolated from strain PsF84 by using chromatographic techniques. The molecular formula of the antifungal VOC was deduced to be C14H22O by EI-MS and ID and 2D NMR spectral analysis. 2,4-Di-tert-butylphenol was found to be effective against an agriculturally important fungus, namely, F. oxysporum, in inhibiting spore germination and hyphal growth. Molecular docking analysis of 2,4-di-tert-butylphenol with beta-tubulin further validated the potential of beta-tubulin binding in F. oxysporum. Two residues of beta-tubulin protein, HIS 118 and THR 117, showed hydrogen binding with ligand. To the authors' knowledge, this is the first report of antifungal VOC (2,4-di-tert-butylphenol) produced by P. monteilii PsF84 that can be a potent inhibitor of beta-tubulin of F. oxysporum.
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