期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 19, 页码 4392-4398出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf5014555
关键词
H-1 NMR; carrots; consensus PCA; metabolite extraction
资金
- Danish Research Council FTP [274-09-0169]
- Ministry of Food, Agriculture and Fisheries [3304-FVFP-08-K-04-01]
Genetic variation is the most influential factor for carrot (Daucus carota L.) composition. However, difference in metabolite content between carrot varieties has not been described by NMR, although primary metabolites are important for human health and sensory properties. The aim of the present study was to investigate the effect of genotype on carrot metabolite composition using a H-1 NMR-based metabolomics approach. After extraction using aqueous and organic solvents, 25 hydrophilic metabolites, beta-carotene, sterols, triacylglycerols, and phospholipids were detected. Multiblock PCA showed that three principal components could be identified for classification of the five carrot varieties using different spectroscopic regions and the results of the two solvent extraction methods as blocks. The varieties were characterized by differences in carbohydrate, amino acid, nucleotide, fatty acid, sterol, and beta-carotene contents. H-1 NMR spectroscopy coupled with multiblock data analysis was an efficient and useful tool to map the carrot metabolome and identify genetic differences between varieties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据