期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 2, 页码 454-461出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf404140v
关键词
lipase-catalyzed acidolysis; p-coumaric acid; seal blubber oil; menhaden oil; phenolipid; DPPH radical scavenging activity; oxygen radical absorbance capacity; reducing power; beta-carotene-linoleate; human LDL cholesterol; DNA
资金
- Natural Sciences and Engineering Research Council (NSERC) of Canada
Lipase-catalyzed acidolysis of p-coumaric acid with seal blubber oil (SBO) and menhaden oil (MHO) was carried Out, followed by identification of major phenolipids in the resultant acidolysis mixture using high-performance liquid chromatography/mass spectrometry. Separation of phenolipid components from the resultant acidolysis mixture was achieved using flash column chromatography. The antioxidant activities of the phenolipids were examined in in vitro assays and biological model systems. The major phenolipids identified from acidolysis mixtures with both SBO and MHO included eight phenolic monoacylglycerols and eight phenolic diacylglycerols. Phenolipids derived from SBO and MHO generally showed good antioxidant potential in the systems tested. The prepared phenolipids exhibited high scavenging capacity toward 1,1-diphenyl-2-picrylhydrazyl (DPPH) and peroxyl radicals and displayed reducing power, strong inhibitory effect on bleaching of beta-carotene, human low-density lipoprotein (LDL) cholesterol oxidation, as well as radical-induced DNA cleavage, thus suggesting that phenolipids derived from omega-3 oils may be used as potential stable products for health promotion and disease risk reduction.
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