期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 11, 页码 2625-2630出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf304997r
关键词
phosmet; surface plasmon resonance; cd-ELISA; cross reactivity
资金
- Natural Science Foundation of Tianjin [13JCQNJC14800]
- National Spark Program [S2012A100036]
- Dr. Foundation of Tianjin Normal University [52XB1103]
A surface plasmon resonance (SPR) immunoassay using a PH-BSA immobilized sensor chip was developed to measure phosmet in food samples. The in competitive inhibition assay showed highly sensitive and good specificity of the cross-reactivity with analogue structure pesticides. The biosensor exhibited a linear detection range from 8.0 to 600 ng/L of phosmet with a lower detection limit of 1.6 ng/L (S/N = 3). The sensitivity obtained with the present SPR affinity biosensor was significantly higher than most of the sensors reported with different measurement methodologies for phosmet. A recovery test of pesticide quantification in peaches, apples, cabbages, and rapes was also studied. Good recoveries (86A-102.8%) and coefficients of variation (CVs) (5.1-12.6%) were obtained in all cases. The SPR biosensor assay method was compared with cd-ELISA in terms of analysis time, antibody dosage, recoveries, precision, detection limit, pretreatment, and testing costs, and clear advantages could be seen over the traditional ELISA-based detection systems. The developed SPR method was suitable for the rapid quantitative or qualitative determination of phosmet in agricultural products.
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