期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 11, 页码 2709-2719出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf305497r
关键词
amino acid; Ziziphus jujuba; hydrophilic interaction chromatography; ultra-high-performance liquid chromatography; triple-quadrupole mass spectrometry
资金
- National Natural Science Foundation of China [30672678]
- Youth Natural Science Foundation of Nanjing University of Chinese Medicine [12XZR01]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [ysxk-2010]
- Ministry of Education [NCET-09-0163]
In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm X 100 mm, 1.7 mu m) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC-UHPLC-TQ:MS is an effective technique to analyze underivatized amino acids in the food samples.
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