期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 13, 页码 3186-3192出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf400912s
关键词
lignin distribution; internode development; stained stem cross section; image analysis; color quantification
资金
- French National Research Agency (ANR) [ANR-08-BLAN-0307-01]
- INRA CEPIA
- INRA BV
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0307] Funding Source: Agence Nationale de la Recherche (ANR)
This work presents a method to quantify the lignification of maize tissues by automated color image analysis of stained maize stem cross sections. Safranin and Alcian blue staining makes lignified tissues appear red, and nonlignified tissues appear blue. Lignification is assessed by the ratio of red intensity over blue intensity. A rough quantification of global lignification is computed as the surface ratio of lignified tissues. A more precise quantification is obtained by computing profiles of red/blue intensity ratio in relation to the distance to the epidermis, depicting the spatial distribution of lignified walls within the stem. Lignification profiles are analyzed through summary parameters describing the evolution of lignification in three specific regions. The distribution of lignification can be quickly assessed depending on the position and the development stage, allowing the screening of genetic variations to be envisioned.
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