期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 43, 页码 10398-10407出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf4026103
关键词
postharvesting process; plant metabolomics; Astragalus root; quality assessment
资金
- National Research Foundation of Korea
- Korean government (MSIP) (University-Institute cooperation program)
- Korea Basic Science Institute [T3373B]
A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus roots with a focus on the peeling procedure using H-1 NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the H-1 NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus roots. Peeled roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus root. These results demonstrate that metabolite profiling based on a combination of H-1 NMR and UPLC-MS analyses can be used to evaluate peeling procedures used in the postharvest processing of herbal medicines.
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