期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 4, 页码 973-978出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf204720q
关键词
dipeptidyl-peptidase IV inhibitor; Atlantic salmon; gelatin; type 2 diabetes; bioactive peptide
资金
- China Medical University [CMU 99-S-37]
The dipeptidyl-peptidase IV (DPP-W)-inhibitory activity of peptides derived from Atlantic salmon skin gelatin hydrolyzed by alcalase (ALA), bromelain (BRO), and Flavourzyme (FLA) was determined. The FLA hydrolysate with the enzyme/substrate ratio of 6% showed the greatest DPP-IV-inhibitory activity. The hydrolysate was fractionated by ultrafiltration with 1 and 2.5 kDa cutoff membranes, and the <1 kDa fraction had the highest DPP-IV-inhibitory activity with an IC50 value of 1.35 mg/mL. The F-1 fraction further isolated by HPLC showed the IC50 value against DPP-IV of 57.3 mu g/mL, and the peptide sequences were identified as Gly-Pro-Ala-Glu (372.4 Da) and Gly-Pro-Gly-Ala (300.4 Da). The synthetic peptides showed dose-dependent inhibition effects on DPP-IV with IC50 values of 49.6 and 41.9 mu M, respectively. The results suggest that the peptides derived from Atlantic salmon skin gelatin would be beneficial ingredients for functional foods or pharmaceuticals against type 2 diabetes.
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