期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 34, 页码 8450-8456出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf3031315
关键词
purification; adsorption; decoloration; deproteinization; polysaccharide; macroporous resin
资金
- National Natural Science Foundation of China [30671469]
A novel method for the purification of crude polysaccharide from fermentation broth of pumpkin residues by macroporous resins was developed. Through static adsorption and desorption and adsorption kinetic tests, xix resins (AB-8, S-8, HPH480, HPD100, X-5, and D101) with different polarity, diameter, and surface area were studied for simultaneous decoloration and deproteinization of crude polysaccharide, and S-8 was chosen as the best one Dynamic breakthrough and desorption tests were performed in a glass column packed with S-8 resin, and the resulting adsorption ratios of pigment and protein were 84.3% and 75.9% (w/w),respectively, with a recovery ratio of polysaccharide 84.7% (w/w). S-8 resin also exhibited higher purification efficiency than the other tested traditional methods Moreover, UV/vis spectroscopy (200-900 nm), analysis revealed most Of the pigment and protein were absorbed by S-8 resin, and HPLC. (containing a refractive index detector and a HPSEC column) results indicated that there was no degradation of the polysaccharide. This automated and efficient method via adsorption-desorption strategy could have potential in scale up :purification and preparation of polysaccharide in the future
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