期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 36, 页码 9120-9129出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf3022018
关键词
Ecklonia stolonifera; anti-inflammation; NF-kappa B; MAP kinases; RAW 264.7 cells
资金
- Fishery Commercialization Technology Development Program of the Ministry for Food, Agriculture, Forestry and Fisheries [iPET109200-3]
- Brain Busan 21 Program, Republic of Korea
Ecklonia stolonifera is a brown alga that was shown to have antioxidant, anti-inflammatory, tyrosinase inhibitory, and chemopreventive activities. However, the molecular mechanisms underlying its anti-inflammatory activity remain unclear. In this study, we investigated the molecular mechanism of the anti-inflammatory action of E. stolonifera ethanolic extracts (ESE) using lipopolysaccharide (LPS)-stimulated RkW 264.7 cells. ESE inhibited LPS-induced nitric oxide (IC50 = 72 +/- 1.9 mu g/mL) and prostaglandin E-2 (IC50 = 98 +/- 5.3 mu g/mL) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. ESE also reduced the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. LPS-induced nuclear factor-kappa B (NF-kappa B) transcriptional activity and NF-kappa B translocation into the nucleus were significantly inhibited by ESE treatment through the prevention of the degradation of inhibitor kappa B-alpha. Moreover, ESE inhibited the activation of Akt, ERK, JNK1/2, and p38 MAPK in LPS-stimulated RAW 264.7 cells. The main components with anti-inflammatory activity in ESE were identified as phlorofucofuroeckol A and B based on the inhibition of NO production. Our results indicate that ESE can be considered as a potential source of therapeutic agents for inflammatory diseases.
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