期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 27, 页码 6839-6845出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf301392y
关键词
prebiotic; probiotic; Lactobacillus; galactooligosaccharides
资金
- Junta de Castilla-La Mancha
- European regional development fund (ERDF) [POII10-0178-4685]
- Comunidad Autonoma de Madrid [ALIBIRD S2009/AGR-1469]
- Ministerio de Ciencia e Innovacion [AGL2011-27884]
- USDA Food Safety Consortium
- USDA-NIFSI [2008-51110-04339]
- CSIC
In this study bioactive caseinomacropeptide was conjugated with prebiotic galactooligosaccharides (hCMP:GOS) by Maillard reaction to synthesize value added prebiotic compounds to Lactobacillus strains. Growth study showed the ability of hCMP:GOS to serve as a sole carbon source for Lactobacillus strains. A significant amount of acetate and lactate was detected in cell free culture supernatant by HPLC. It demonstrated the ability of Lactobacillus strains to ferment the hCMP:GOS as a carbon source. In addition, hCMP:GOS grown Lactobacillus cells exhibited enhanced bile tolerance and retained 90% viability. Overall results of this study indicate that the hCMP conjugated GOS can be potential multipurpose prebiotic substrates to enhance the growth and bile tolerance in Lactobacillus strains and serve as a fermentable substrate to produce beneficial metabolites in the host.
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