期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 41, 页码 10343-10351出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf303124c
关键词
dynamic microwave-assisted extraction; salting-out liquid-liquid extraction; steroid hormone; fish tissue; liquid chromatography-tandem mass spectrometry
资金
- Ministry of Science and Technology of Jilin Province, China [20120327]
- Natural Science Foundation of China [20875037]
In this work, a simple and fast sample pretreatment method was proposed for determination of steroid hormones in fish tissues by coupling dynamic microwave-assisted extraction with salting-out liquid-liquid extraction. The steroid hormones were successively extracted with acetonitrile and water under the action of microwave energy. Subsequently, the extract was separated into an acetonitrile phase and an aqueous phase with ammonium acetate. The acetonitrile phase containing the target analytes was concentrated and determined by LC-MS/MS. The limits of detection for the steroid hormones were in the range of 0.03-0.15 ng g(-1). This method was successfully applied to analyze seven kinds of fish tissues, and the recoveries of the steroid hormones for the spiked samples were in the range of 75.3 +/- 4.9% to 95.4 +/- 6.2%. Compared with the traditional method, the proposed method could reduce the consumption of the organic solvent, shorten the sample preparation time, and increase the sample throughput.
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