期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 4, 页码 896-903出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf202857y
关键词
adulteration; betaines; nonprotein amino acids; screening; tandem mass spectrometry; vegetable oils
资金
- Spanish Ministry of Science and Innovation (Spain) [CTQ2009-09022]
- Comunidad Autonoma of Madrid (Spain) [S2009/AGR-1464]
- FEDER program (ANALISYC-II)
- University of Alcala [CCGIO-UAH/AGR-5950]
- Comunidad Autonoma of Madrid (Spain)
A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five nonprotein amino acids (beta-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in <2 min. The proposed method provided high fingerprinting power to identify the presence of five of the studied compounds in different types of vegetable oils (soybean, sunflower, corn, olive) with LODs at parts per billion levels. The method was validated, and different mixtures of extra virgin olive oil with seed oils were analyzed, achieving the typification for the detection of adulterations in extra virgin olive oils up to 2% w/w. The nonprotein amino acid ornithine was confirmed as a marker for adulteration in the olive oils analyzed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据