期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 18, 页码 4542-4549出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf205266h
关键词
tomato; phenolic metabolites; LC-MS/MS; plasma; urine
资金
- CICYT [AGL2010-22319-C03]
- MAPFRE Foundation
- Spanish Ministry of Science and Innovation (MICINN), Spain [RETICS RD06/0045/0003]
Tomato is a good source of bioactive molecules such as vitamin C, carotenoids, and phenolic compounds. Up to now, only a few studies have evaluated the bioavailability of phenolic compounds from tomato. This paper presents the optimization of a method for the determination of phenolics in tomato and their metabolites in human urine and plasma after ingestion of tomato sauce. The sample preparation includes a SPE step to obtain cleaner extracts for injection in the LC-MS/MS system. The mean recovery of analytes ranged from 73 to 104% in plasma and from 65 to 106% in urine, the accuracy was between 90.3 and 115.0% in urine and between 85.7 and 115.0% in plasma, and the precision coefficient of variation was <15%. The method allowed detection and quantification limits of 0.5-29 and 2.0-90 ng mL(-1) in urine, respectively, and 0.5-30 and 2.0-105 ng mL(-1) in plasma, respectively, for the same phenolic compounds.
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