期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 1, 页码 128-136出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf304546a
关键词
brefeldin A; cardiovascular disease; cell growth; estrogen; Erk1/2; signal transduction
资金
- Special Coordination Fund for Promoting Science and Technology
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Ministry of Economy, Trade and Industry, Japan
- Grants-in-Aid for Scientific Research [23590296] Funding Source: KAKEN
We purified an Erk1/2-activating component in Agaricus blazei and identified it as brefeldin A (BFA). The extract of A. blazei mycelia (ABE) previously showed an estrogenic gene-expression profile and positive effects in patients with cardiovascular symptoms. Here, we demonstrate that BFA has estrogenic activity in reporter gene assays and stimulates an estrogen-receptor pathway revealed by activation of Erk1/2, although BFA had no growth-stimulating activity in breast cancer MCF-7 cells. The presence of estrogenic activity without any explicit growth-stimulating effect is unique to BFA, and such components are termed here silent estrogens. To test this hypothesis, we examined the target-gene transcription and signaling pathways induced by BFA. Furthermore, BFA was found in the mycelium but not fruiting body of A. blazei, suggesting the potential use of ABE for therapeutics and its supplementary use in traditional medicines and functional foods.
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