期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 1, 页码 378-385出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf103003q
关键词
Oat beta-glucan; tea polyphenols; equilibrium dialysis; adsorption isotherm; adsorption capacity; response surface methodology
资金
- Chongqing Municipal Commission of Education [2009-2]
Information on interactions between oat beta-glucan and tea polyphenols (TP) is not available in the published literatures. Equilibrium dialysis was applied to determine the adsorption of TP into beta-glucan, and response surface methodology (RSM) was employed to optimize the absorbing variables (pH, temperature, and phosphate buffered saline (PBS) buffer concentration). The equilibrium data at constant temperature were fitted with the Langmuir, Freundlich, and Redlich-Peterson models. The results showed that the Freundlich model was the best method to describe the experimental data. Parabolic curves were obtained for pH and temperature. In terms of adsorption capacity, factors including temperature, pH/temperature, and buffer concentration/temperature had the greatest influence on the response. The highest adsorption capacity of TP into beta-glucan was 134.55 mu g mg(-1) at the following optimized conditions: pH 5.56, PBS buffer concentration 0.13 M, and temperature 40 degrees C. No significant differences (p > 0.05) between the experimental and predicted values confirmed the adequacy of the response surface equations.
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