Acaricidal Activities of Apiol and Its Derivatives from Petroselinum sativum Seeds against Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Tyrophagus putrescentiae

The acaricidal effects of an active constituent derived from Petroselinum sativum seeds and its derivatives were determined using impregnated fabric disk bioassay against Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Tyrophagus putrescentiae and compared with that of synthetic acaricide. The acaricidal constituent of P. sativum was isolated by various chromatographic techniques and identified as apiol. On the basis of LD(50) values against D. farinae and D. pteronyssinus, apiol (0.81 and 0.94 mu g/cm(2)) was 12.4 and 10.2 times more toxic than benzyl benzoate (10.0 and 9.58 mu g/cm(2)), respectively. In acaricidal studies of apiol derivatives, 3,4-methylenedioxybenzonitrile (0.04, 0.03, and 0.59 mu g/cm(2)) was 250, 319, and 20.7 times more toxic than benzyl benzoate (10.0, 9.58, and 12.2 mu g/cm(2)) against D. farinae, D. pteronyssinus, and T. putrescentiae. In structure-activity relationships, the acaricidal activities of apiol derivatives could be related to allyl (-C(3)H(5)) and methoxy (-OCH(3)) functional groups. Furthermore, apiol and its derivatives could be useful for natural acaricides against these three mite species.