期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 12, 页码 6583-6593出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf2006529
关键词
Carrot (Daucus carota); hyperoxia; phenolic antioxidants; postharvest abiotic stresses; reactive oxygen species; wounding
资金
- Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture [2008-34402-19195]
- Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico)
Plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. Here, we report the potential use of carrots (Daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. Wounding stress induced an increase of similar to 287% in total phenolic content (PC) in carrots stored for 48 h at 20 C. This increase was higher (similar to 349%) in the wounded tissue treated with hyperoxia stress. To further understand the physiological role of reactive oxygen species (ROS) as a signaling molecule for the stress-induced accumulation of phenolics in carrots, the respiration rate as well as the enzymatic activities of NADPH oxidase, superoxide dismutase, ascorbate percoxidase, and catalase were evaluated. Likewise, shredded carrots were treated with diphenyleneiodonium chloride solution to block NADPH oxidase ROS productions, and the phenylalanine ammonia lyase activity and total PC were evaluated. Results demonstrated that ROS play a key role as a signaling molecule for the stress-induced accumulation of PC in carrots.
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